Chromatography of amino acids Amino acid Rf value alanine 0.38 arginine 0.20 asparagine 0.5 aspartic acid 0.24 Click to see full answer. In respect to this, how do you find the RF value of an amino acid?The Rf value can be calculated by measuring the distance of the substance from its starting point in millimeters, as well as the distance the solvent traveled from its starting point in millimeters, then dividing the substance distance by the solvent distance.Secondly, why do amino acids have different RF values? The different amino acids move at differing rates on the paper because of differences in their R groups. The rate of movement of a biomolecule during paper chromatography is reported as its relative mobility (Rf). Also, what is the RF value? RF value (in chromatography) The distance travelled by a given component divided by the distance travelled by the solvent front. For a given system at a known temperature, it is a characteristic of the component and can be used to identify components.How does amino acid chromatography work? Chromatographic Separation of Amino acids: The present experiment employs the technique of thin layer chromatography to separate the amino acids in a given mixture. By running controls [known compounds ] alongside, it is possible to identify the components of the mixture.
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